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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in many tumors, which leads to chromosome instability, and so may possibly lead to tumorigenesis. This protein was discovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two extra splice variants have been reported but thorough nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind especially into the target antibody and block antibody binding. These peptide normally consists of the epitope recognized from the antibody.
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This manual outlines additional possible optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This guidebook outlines additional possible optimization of cellular and immunoassay parameters to be sure the best possible outcomes are...
Antibodies certain to the blocking peptide no longer bind into the epitope over the concentrate on protein. By evaluating the staining from the blocked antibody vs . the antibody on your own, you can see which staining is precise.
Focus on Info Cyclin E belongs towards the extremely conserved cyclin family members, whose associates are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E varieties a fancy with and capabilities for a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates within the G1-S period boundary and is also degraded as cells development by way of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...
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